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M. L. Hubert,1 H. Wang,1 H.-W. Chen,1 W. Stryjewski,2 D. Patterson,1,2 M. A. With,1 P. Data,1 J. Getter,1 M. C. Murphy,1 and S. A. Soper1,2 1 A FIELD-DEPLOYABLE SYSTEM FOR AUTOMATED MOLECULAR TESTING

We developed a highly accurate, stable endoscopic scale system, which has the following features: (1) a small, open-loop system with a small, single injection port that can be rapidly modified to accommodate complex, multi-dose solutions; (2) a user-programmable system that enables the user to fine tune the conditions that must occur before an assay can occur; (3) the ability to process any single, or a combination of multiple, samples without requiring preprocessing; and (4) an automatic calibration with a real-time system or via an automated, self-calibration system via an optical sensor (Fig. 1). A sample was defined as a single, non-migrated, cell, that is, the cell was removed from the liquid to the point where no water was present. The sample was separated from the cell matrix by a non-polarizable poly dextrose membrane. Each sample was subjected to a second, small, open-loop system, which comprises a pump consisting of a sealed, self-contained, pump-injection port that can accommodate multiple samples, to provide sample-to-sample and/or flow-rate-to-sample exchange. Once the sample entered the pump, it was injected with a concentration of a known or prepared reagent and an appropriate volume of fluid is flowed to the sample, in order to form a gel. The pump delivers the fluid to the probe, which is then attached to the sample. The probe was introduced into the system as a fully-assembled sample collection system (Fig. 2A). A second open-loop system, in which sample flow is delivered to the probe through an autotransformable, flexible tubing, was used to assess the efficacy of a series of immunoassay procedures (Fig. 2B–2D). We performed three different tests on the three different types of cells, with different concentrations and mixtures of reagents. Each test requires a few seconds, and a series of independent assays can be performed simultaneously. Fig. 1 Characterization and system design.

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A field-deployable system is designed to be quickly and easily set up in an outdoor environment for various purposes such as military operations, emergency response, scientific research, and exploration.

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The requirement to file a field-deployable system depends on the specific regulations and policies of the relevant governing bodies or organizations. Generally, it can be mandatory for military agencies, government entities, research institutions, or any group that plans to deploy such systems in specific locations.

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The purpose of a field-deployable system is to enable effective operations or research in outdoor environments. It allows for rapid setup, mobility, and functionality in remote or challenging locations where traditional infrastructure may not be available or feasible.

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The information reported on a field-deployable system can include details about the system's design, functionality, intended use, deployment location, personnel involved, safety protocols, environmental impact, and any necessary permits or certifications.

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