These discrepancies have been attributed to the limitations associated with using a retro viral vector which lacks a retro viral envelope nor a genome-editing facility (P.A. Johnson and D.A. Williams Recombinant retro viral vectors have been used to transfer a variety of genetic sequences into hematopoietic stem cells. Although transfer and expression of foreign genetic sequences into reconstituting stem cells is one approach to somatic gene therapy, few studies have shown long-lasting phenotypic changes in recipient mice in vivo. These discrepancies have been attributed to the limitations associated with using a retro viral vector which lacks a retro viral envelope nor a genome-editing facility (P.A. Johnson, D.A. Williams, and S.G. Moore, unpublished observations). Here we employ a recombinant retro viral vector encoding a retro viral envelope containing the HIV Tat protein and a genomic marker encoding a chimeric-GFP fusion with a constitutive G protein (GREW). Two generations of GFP-GRG? chimeras were generated: GAP(Tat) -Tat chimeric/GAP(Gr) -Tat chimeric and Gupta(Gr) -Tat(Gr) chimeric/GAP(Gr) chimeric. After 2 weeks of expression in HEK-293T-B6 HCT116 cells, a GAP marker was generated from HCT116 cells, and the cells were treated to introduce a retro viral envelope. After 30 days, the cells were harvested and the transduced progeny were challenged with a lethal dose of lentiviruses. All chimeras were protected from all antiretroviral (ARV)-associated cytotoxicity tests at 0.01 ml/mouse injected into the tail vein of each mouse. The same strategy of transducing transduced cells with different vector proteins and/or gene expression schemes was used to generate chimeric GAP(Gr) -Tat(Gr) chimeras. These chimeras had reduced GAP expression in the tail vein of recipient mice compared to the respective control(s), demonstrating the efficacy of the chimeric Gupta(Gr) chimeric/GAP(Gr) -Tat(Gr) chimeric approach in vivo.
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Serial Transplantation of -Resistant Bone Marrow: Protection of Murine Recipients from Drug Toxicity by Progeny of Transduced Stem Cells C.A. Corey, A.D. Silva, C.A. Holland, and D.A. Williams Recombinant
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What is serial transplantation of -resistant?
Serial transplantation of -resistant refers to the process of repeatedly transplanting a substance or graft that is resistant to a particular condition or treatment.
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The process for filling out serial transplantation of -resistant forms or documentation may differ depending on the specific requirements or guidelines provided by the responsible authority or organization. However, it generally involves providing detailed information about the resistant substance or graft, transplantation procedures, relevant outcomes, and any additional supporting data or research.
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The purpose of serial transplantation of -resistant is to investigate or study the behavior, effectiveness, or characteristics of substances or grafts that display resistance to a specific condition or treatment. It aims to gain a better understanding of resistance mechanisms, potential therapeutic approaches, or possible advancements in transplantation procedures.
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The specific information that must be reported on serial transplantation of -resistant can vary depending on the regulations, guidelines, or research requirements in place. Generally, it may include details such as the nature of the resistant substance or graft, transplantation protocols, observed outcomes, experimental design, statistical analysis, and any other relevant findings or conclusions.
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