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University of PennsylvaniaScholarlyCommons Publicly Accessible Penn Dissertations 2014A ChemicalGenetic Screen for Identifying Substrates of the Er Kinase Perk Nancy L. Mass University of Pennsylvania,
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How to fill out a chemical-genetic screen for

01
Define the specific question or hypothesis you want to address through the chemical-genetic screen.
02
Select a suitable model organism for the screen, such as yeast, worms, or fruit flies.
03
Choose a relevant library of chemical compounds to test, ensuring it covers a wide range of potential targets.
04
Design an assay that can effectively measure the phenotype of interest in response to chemical exposure.
05
Conduct the screen by exposing the organism to the chemicals and analyzing the resulting phenotypic changes.
06
Validate hits from the screen through follow-up experiments and data analysis.

Who needs a chemical-genetic screen for?

01
Researchers studying gene function and interaction.
02
Scientists investigating drug targets and molecular pathways.
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Pharmaceutical companies looking for new therapeutic compounds.
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Biotech companies developing novel treatments or diagnostics.

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