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CHAPTER 3. LABORATORY FOURIER TRANSFORM INFRARED SPECTROSCOPY METHODS FOR GEOLOGIC SAMPLES P.L. King1, M.S. Ramsey2, P.F. McMillan3, 4, & G. Swayze5 1 Department of Earth Sciences, University of Western

Center for Nanotechnology, University of Central Lancashire, Preston on Avon L1 5PE, United Kingdom 5Department of Physiology, University of Southampton, Southampton LS4 1QS, United Kingdom 5(7) Nature, DOI: 10.1038/nature09947 ABSTRACT. The laboratory fitter transgenic rabbit model was used to study the effect of different genetic manipulations of mammalian chromosome 2 on genomic stability. The effects of fitter and transgenic loci were compared to those of wild type rabbit loci using the stability assay and the DNA content was measured in loci. The two classes of fitter vectors (LF-2 and RF2) significantly decreased the level of genomic instability in the loci of the fitter genotype, whereas transgenic loci could not reproduce the decrease. No differences between fitter and transgenic loci were observed in the results of the DNA contents of genomic loci. The observed decrease in genomic instability with fitter transgenic loci suggested that fitter transgenic loci would be more suitable as laboratory fitter vectors, because such vectors were more stable. Keywords The lab fitter (LF-2 or RF2) and transgenic rabbit (LF-2) models were designed to provide transgenic and fitter loci, respectively, for chromosome 2 research in animal models. The laboratory fitter loci are composed of loci containing the transgenic or fitter allele and those loci containing the wild Type II allele of mouse chromosome 2 (Lane et al., 1998). Both types of fitter loci were created from a single Gemini CDA library containing 8.9 × 107 genomic loci from 1 rabbit. The two classes of wild type fitter loci (LF-1 and LF-2) were created from a single Gemini CDA library composed of 1 × 107 genomic loci obtained from a single rabbit. The 2-fold difference in DNA volume between lab fitter and wild type loci made it difficult to compare effects of the different genetic manipulations because they are not identical in the number of genomic loci.

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Laboratory Fourier Transform Infrared (FTIR) is a technique used for the identification and analysis of chemical compounds. It involves measuring the absorption of infrared radiation by a sample, allowing the identification of functional groups and molecular structures.

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The requirement to file Laboratory Fourier Transform Infrared (FTIR) can vary depending on the specific regulations or guidelines of the governing body or organization. Generally, it is required to be filed by laboratories or researchers conducting chemical analysis and identification using FTIR spectroscopy techniques.

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The process of filling out a Laboratory Fourier Transform Infrared (FTIR) report may depend on the specific requirements or guidelines provided by the governing body or organization. Typically, it involves gathering the necessary sample information, conducting the FTIR analysis, and documenting the results and any additional relevant details in the designated report or form.

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The purpose of Laboratory Fourier Transform Infrared (FTIR) is to analyze and identify chemical compounds present in a sample. It can be used for various purposes, including quality control, forensic analysis, environmental monitoring, pharmaceutical analysis, and material characterization.

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The specific information that needs to be reported on a Laboratory Fourier Transform Infrared (FTIR) analysis can vary based on the nature of the analysis and the requirements set by the governing body or organization. Generally, it includes details such as sample identification, analysis conditions, spectra interpretation, and any relevant observations or conclusions.

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