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Pretty good so far. Was a little surprised that I had to pay once all the work was done. But its okay, have used it a couple of times and its working.
2016-06-14
PDFfiller is so user friendly. It helps immensely as a private practice owner where I need to fill-in pdf documents, convert to other formats, and insert verified signatures. I would recommend to other business owners.
2018-04-25
Impressive features and easy to use. My only gripe is that there appears to be no way of changing the date function to day/month/year, but it's just as easy to enter the date manually
2019-03-22
I'm doing my own real estate contract and pdffiller has made this experience less stressful and actually kind of fun! I'll definitely be using this service a lot.
2019-09-10
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Sometimes the system will not allow me to download the forms I've edited. Sometimes there are glitches in the system that prevent us from logging in and saving documents. Sometimes system can be a bit slow, I notice it more in the afternoon where people may use the system more.
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Make sure to explore all functions this product offers! There is a ton of functions for the price you pay, take full advantage and improve your day to day work!
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I am making documents more user friendly for my clients. This helps us retain business for ease of paperwork completion.
I like that the system is user friendly. It makes the paperwork process for our clients easier which helps us win and retain a lot of business.
What do you dislike?
Sometimes the system will not allow me to download the forms I've edited. Sometimes there are glitches in the system that prevent us from logging in and saving documents. Sometimes system can be a bit slow, I notice it more in the afternoon where people may use the system more.
Recommendations to others considering the product:
Make sure to explore all functions this product offers! There is a ton of functions for the price you pay, take full advantage and improve your day to day work!
What problems are you solving with the product? What benefits have you realized?
I am making documents more user friendly for my clients. This helps us retain business for ease of paperwork completion.
2019-05-21
Great service that has a lot to offer…
Great service that has a lot to offer besides editing pdf files. Love this and it has been well worth the money.
2020-02-03
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2024-04-25
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2023-07-04
I found myself trying to rate it 4…
I found myself trying to rate it 4 stars, but it's honest about what it is- a robust, web-based pdf editing tool that doesn't try to be more than it is, and does what it is supposed to do very well.
2022-01-25
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How much DNA do you need for transformation?
For successful chemical transformation, 50100 SL of competent cells and 110 NG of DNA are recommended. When a ligation mixture is used as the transforming DNA (often 15 All is sufficient), purification prior to chemical transformation is generally not required.
How much DNA do you add to transformation?
High Efficiency Transformation Protocol Add 1 pg-100 NG of plasmid DNA (1-5 SL) to cells and mix without voting.
How much DNA do you add to electroporating?
Once DNA is added to the cells, electroporating can be carried out immediately. It is not necessary to incubate DNA with cells. The maximum recommended volume of a DNA solution to be added is 2.5 SL.
What is a good transformation efficiency?
A good rule to follow is this: if your efficiency is equal to or less than 5 × 107 CFU/kg DNA, use these cells for plasmid transformations. If your efficiency is greater than 5 × 107 (ideally 1 × 108 or higher), use these cells for ligation and other assembly reaction transformations.
What is required for transformation?
The process of gene transfer by transformation does not require a living donor cell but only requires the presence of persistent DNA in the environment. The prerequisite for bacteria to undergo transformation is its ability to take up free, extracellular genetic material. Such bacteria are termed as competent cells.
How much plasmid do I need for transformation?
Most recent answer. When you get at least 100 CFU from a transformation made with 0.1 ng of circular plasmid (ideally pUC18 or pUC19). This level of competency (106 CFU/kg of DNA) is the lower limit required for efficient cloning purpose.
What is a plasmid transformation?
Plasmid or vector transformation is the process by which exogenous DNA is transferred into the host cell. Transformation usually implies uptake of DNA into bacterial, yeast or plant cells, while transfection is a term usually reserved for mammalian cells.
What is a good transformation efficiency value?
A good rule to follow is this: if your efficiency is equal to or less than 5 × 107 CFU/kg DNA, use these cells for plasmid transformations. If your efficiency is greater than 5 × 107 (ideally 1 × 108 or higher), use these cells for ligation and other assembly reaction transformations.
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