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Affordable, reliable, and flexible with changes. Could use a little more explaining to become more user friendly without having to get technical assistance all the time.
2017-08-14
It's very good and I recommend it to others. I know I am not getting the full benefit, and should attend a webinar but feel too busy unless it could be archived and watched at my convenience.
2018-03-07
Quick and easy editing features. Not sure how to utilized the signing of documents feature, but will figure it out. I also would like to delete old docs no longer needed. An online class for the software use would be appreciated.
2018-04-30
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Being able to type into a form that would otherwise be hard to complete.
What do you dislike?
Once document is finished, it is always like a Easter egg hunt to find its saved location on my PC. Should be in downloads folder, but guess again!
What problems are you solving with the product? What benefits have you realized?
PDF filler allows one to complete a complicated form in legible printed characters. This makes a very good impression with clients, banks and other recipients of the finished document.
2018-12-22
Positive review
It is so much easier to get my scholarships done. I hate writing on my scholarships, so I was having to completely retype the application which was taking time from me filling them out.One suggestion would be to do a student discount, like in my case using it for scholarships. A way to do this could be they have to enter there school and get a school code from someone and then that could verify they are a student so the company is not getting taken advantage of.
2020-03-22
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2019-03-12
Honestly, I used this service for one day. I did the whole hoopla, signing up for the service, getting my PDF's and documents taken care of, then went in to cancel my free trial. Well, something went wrong, and I was charged for a full year that I didn't want. But the reason they get 5 stars instead of zero-- they voided the charge same day that I emailed them. They weren't worried about pinching pennies, they were worried about being a good business and doing the right thing. That's a 10/10 in my book. Honestly, I have them bookmarked on my webpage so that if/when I need their services again, I'll definitely be going back!
2023-08-23
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2020-11-06
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How is transformation efficiency calculated?
Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. It can be calculated by dividing the number of successful transform ants by the amount of DNA used during a transformation procedure.
What is a normal transformation efficiency?
This is divided by the amount of DNA used in the transformation and expressed as transform ants per microgram of DNA. Transformation efficiencies between 10^6 and 10^9 represent the normal range for competent E. coli cells.
What is a good transformation efficiency?
A good rule to follow is this: if your efficiency is equal to or less than 5 × 107 CFU/kg DNA, use these cells for plasmid transformations. If your efficiency is greater than 5 × 107 (ideally 1 × 108 or higher), use these cells for ligation and other assembly reaction transformations.
What is the transformation efficiency of E cold?
cold (Subcloning Efficiency)? Transformation efficiency is defined as the number of colony forming units (CFU) which would be produced by transforming 1 kg of plasmid into a given volume of competent cells. The term is somewhat misleading in that 1 AEG of plasmid is rarely actually transformed.
How is transformation efficiency calculated polo?
Transformation efficiency = Total number of colonies growing on the agar plate. Amount of DNA spread on the agar plate (in kg) Enter that number here Total number of colonies = (DNA in AEG) = (concentration of DNA in AEG/All) x (volume of DNA in All) Enter that number here. Total amount of polo DNA, AEG.
How does plasmid size affect transformation efficiency?
The transformation efficiency (transform ants per microgram plasmid DNA) decreased with increases of size of the DNA. The size of plasmid DNA in the range of 3.7 to 12.6 KB did not affect the molecular efficiency (transform ants per molecule input DNA).
Why are vials incubated for 10 minutes?
To make the cells competent by making the cell membranes more permeable to DNA. Why were the vials incubated for 10 minutes in LB broth rather than transferring their contents directly to the plates? To allow cells time to produce the gene for ampicillin resistance.
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