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What is resolution formula?

Resolution (r) = 1.22/(NA(obj) + NA(cold)) Where r is resolution (the smallest resolvable distance between two objects), NA is a general term for the microscope numerical aperture, is the imaging wavelength, NA(obj) equals the objective numerical aperture, and NA(cold) is the condenser numerical aperture.

What is the formula to calculate resolution?

Equation (1) indicates that the resolution is the difference between peak retention times divided by the average peak width. In a peak with Gaussian distribution, the peak width is W = 4 (where is the standard deviation) and the peak FHM is W0. 5h = 2.354.

What is resolution in HPLC formula?

Resolution. The resolution of an elation is a quantitative measure of how well two elation peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elation peaks.

How do you calculate peak width in HPLC?

The width at half-height is determined by measuring the height of the peak crest above the baseline, dividing by two, and then measuring the span between the rising and falling sides of the peak where the signal crosses the half-height points.

What does resolution mean in chromatography?

In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.

How do you calculate column efficiency?

Column efficiency, indicated as the number of theoretical plates per column, is calculated as N = 5.54 (tR / w0.5)2 where tRy is the retention time of the analyte of interest and w0.5 the width of the peak at half height.

How do you determine the resolution of a microscope?

NA= n x sin Where n is the refractive index of the imaging medium and is half of the angular aperture of the objective. D= /2 NA. Where is the wavelength of light used to image a specimen. D= 2 /NA2 R= 1.22 /Naomi+Second.

How do you calculate resolving power?

= wavelength. U = angle of the cone of light coming from object. U' = angle of cone of light forming image. N = refraction index of object. M = magnification. NA = numerical aperture. D = distance between two points in the image. D = 0.61 / (m tank') (1)* **

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